REPLICATION DETECTIVE

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Replication Fiber Lab SINGLE-MOLECULE LAB

SIMULATION-FIRST PROTOTYPE

Can we make replication visibly mechanistic?

Follow one DNA molecule as MCM loads, replication begins at selected sites, and forks move through S phase.

Replication simulation

Licensing

MCM double hexamers are loading

An illustrative molecule will begin automatically.

0loaded MCM
0origins fired
0%DNA copied
PLAYBACK
Playback speed
Replication across one illustrative DNA molecule Playback controls sit above the regulatory activity plot. The live molecule sits above its aligned kymograph at the same genomic scale. Loaded MCM appears only at sites used in the current realization.

A new seeded realization is sampled for each molecule

Loaded MCMstationary double hexamer
Active CMGCMG at a moving fork
Replicated DNAtwo daughter fibers
Initiationloaded MCM becomes two moving CMGs
Terminationconverging forks complete intervening DNA
×Passive replicationincoming fork reaches loaded origin
Stalled forkstationary CMG at a fixed position

REPLICATION DETECTIVE

What do these symbols correspond to?

Use the live molecule and kymograph to test each interpretation independently.

×

What does the grey × mark?

What does the red symbol mark?

Where does initiation take place?

Where does termination take place?

REPLICATION SEQUENCE

From loading to completion

  1. 01LicenseMCM loads at multiple sites
  2. 02InitiateA subset becomes CMG
  3. 03ElongateBidirectional bubbles grow
  4. 04TerminateConverging forks finish the intervening DNA

Replication Fiber Lab

The animation shows MCM loading, fork movement, and completion across one DNA molecule.